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KMID : 1134820220510030221
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Journal of the Korean Society of Food Science and Nutrition
2022 Volume.51 No. 3 p.221 ~ p.228
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Apoptotic Effects of Butanol Fraction of Lysimachia foenum-graecum by Regulating Akt/mTOR Signaling Pathways in AGS Gastric Cancer Cells
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Lee Hyo-Jae
Seo Ji-Won
Kim Sang-Hyeon
Lee Chang-Yeol
Kim Sang-Yong
Kim Young-Min
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Abstract
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Lysimachia foenum-graecum is a herbal plant known to be effective in relieving headaches, toothaches, and inflammation. It is known to have antioxidant, anti-obesity, and anti-inflammatory effects. However, anti-gastric cancer effects have so far not been reported. The purpose of this study was to investigate the apoptotic effects of the butanol fraction of Lysimachia foenum-graecum (LFB) in AGS human gastric cancer cells. The antiproliferative activity of LFB was confirmed by MTT analysis, and the apoptotic effect was confirmed by Annexin-V analysis. In addition, it was confirmed through western blotting that the LFB treatment reduced the expression levels of p-Akt and p-mTOR in a concentration-dependent manner. Akt plays a role in cell proliferation and migration of cancer cells by regulating important signaling factors such as mTOR and p53. mTOR induces the ubiquitination of p53 and plays an important role in tumorigenesis and the survival of cancer cells. This inhibition of Akt and mTOR leads to apoptosis by regulating the signaling molecules of the intrinsic pathway. In addition, LFB increases the expression level of the effector caspase, caspase-3, in a concentration-dependent manner. The expression of apoptosis-related signal proteins was confirmed by treatment with LY2940002 (Akt inhibitor) and rapamycin (mTOR inhibitor). These results indicate that LFB induces apoptosis through the endogenous pathway by suppressing the expression of Akt and mTOR.
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KEYWORD
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AGS, Akt/mTOR pathway, apoptosis, intrinsic pathway, Lysimachia foenum-graecum
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